CORDIS Project
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The project aims to create artificial metalloenzymes using SPY technology for photostimulated reactions. By fusing specific proteins with metal-containing peptides, the goal is to develop new biocatalysts that can be utilized in various industrial applications.
Protein redesign allows to devise enzymes with new structure and functions, however, the introduction of artificial metal cofactors to a protein and tailoring the second coordination sphere of the metal centre remains challenging. SPY technology is a way to tackle these problems.In U-SPY this technology will be used to devise new artificial metalloenzymes capable for photostimulated reactions.
This tagging system has been widely used but no examples are reported about SPY-based metalloenzymes. T…
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