CORDIS Project
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This project focuses on engineering RNA-guided proteins for precise genetic manipulation. It aims to develop tools for RNA editing that can correct genetic mutations, with potential applications in treating diseases and studying protein functions.
Enzymatically active RNA-guided proteins, like the RNA-induced silencing complex (RISC), are particularly versatile tools for the rationally programmed manipulation of genetic information.
After successful re-addressing of various natural RNA-guided machineries it is now time to tackle the engineering of novel, user-defined tools.
With this respect we have recently achieved the engineering of an RNA-guided adenosine-to-inosine RNA editing machinery.
Since inosine is biochemically read as guanosi…
EBERHARD KARLS UNIVERSITAET TUEBINGEN
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