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The NASPA project develops a versatile nucleic acid detection method using short prokaryotic Argonautes for point-of-care diagnostics. This one-pot technique allows for the detection of specific DNA sequences with a fluorescent signal, overcoming limitations of traditional PCR methods.
The SARS-CoV-2 pandemic highlighted the need for versatile diagnostics tools.
Detecting presence of specific nucleic acid sequences is a powerful method for pathogen diagnostics, phenotyping, and determination of single nucleotide polymorphisms or other mutations underlying genetic diseases. PCR-based methods, the gold standard in nucleic acid detection, require specialized equipment and trained personnel, limiting its applicability at point-of-care or in-field locations.
Alternative isothermal…
WAGENINGEN UNIVERSITY
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