CORDIS Project
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This project aims to develop a synthetic episome using artificial nucleic acids, which is crucial for creating synthetic organisms. It focuses on engineering polymerases and proteins to enable stable replication and maintenance of this episome, paving the way for safer genetically modified organisms.
A long term goal of synthetic biology is the assembly of a cell from its individual components. A genetic element based on synthetic nucleic acids capable of stable propagation, a synthetic episome, is the minimal genetic element required for the systematic development of all cellular components of a synthetic organism based on artificial nucleic acids.
Recent progress in DNA polymerase engineering has successfully isolated variants with expanded substrate spectra capable of efficiently synthesi…
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