CORDIS Project
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This project investigates the biosynthetic pathway of 3-acyltetronates by expressing specific enzymes in E. coli. It aims to understand the formation mechanisms of tetronates and their related natural products, potentially enabling the creation of novel compounds through combinatorial biosynthesis.
A main aim of the research project is the elucidation of a general biosynthetic pathway of 3-acyltetronates.
For this purpose, enzymes, which were identified in the gene clusters of the phosphatase inhibitor RK-682 and the quasi-enantiomeric ionophoric polyethers tetronasin and tetronomycin to be potentially responsible for tetronate formation will be heterologously expressed in E.coli.
The purified enzymes will be applied for the development of in vitro assays with synthetically prepared surrog…
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