CORDIS Project
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This project investigates how pulsed electric fields can enhance the delivery of DNA into cells, aiming to improve gene therapy for cancer and infectious diseases. By understanding the physical mechanisms involved, the project seeks to optimize the efficiency of gene transfer techniques in various tissue environments.
Application of pulsed electric field (PEF) can reversibly increase the permeability of the cell membrane allowing the access of otherwise impermeable DNA molecules to the inside of the cell.
Introduction of foreign DNA molecules encoding immuno-modulatory proteins, antibodies and antigens into cells using PEF, known as Gene Electro-Transfer (GET), is increasingly used for the modulation of the immune system or immunotherapy.
While GET based immunotherapy presents itself as a potent application f…
UNIVERZA V LJUBLJANI
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