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The GENEXP project aims to develop a comprehensive system for analyzing gene expression kinetics of endogenous genes in human cells. It focuses on tagging genes and their mRNAs to study transcription and export processes in live cells, providing insights into gene regulation within their natural context.
Live-cell imaging combined with kinetic analyses has provided new biological insights on the gene expression pathway.
However, such studies in mammalian cells typically require use of exogenous over-expressed gene constructs, which often form large tandem gene arrays and usually lack the complete endogenous regulatory sequences.
It is therefore imperative to design methodology for analyzing gene expression kinetics of single alleles of endogenous genes.
While certain steps have been taken in thi…
BAR ILAN UNIVERSITY
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