CORDIS Project
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This project aims to develop a novel method for tagging proteins in cells to enhance super-resolution microscopy. By integrating genetic code expansion with bio-orthogonal chemistry, it seeks to improve the visualization of specific proteins in various cellular locations.
Super-resolution biological microscopy would benefit from a much smaller alternative to green fluorescent protein and enzyme-mediated labelling methods for imaging specific proteins in cells.
To facilitate routine tagging of organic fluorophores to cellular proteins for super-resolution imaging, we aim to combine genetic code expansion (the most non-invasive tagging method) with cell-compatible CuAAC with copper-chelating azides (the fastest bio-orthogonal reaction) and extend the utilities of t…
MEDICAL RESEARCH COUNCIL
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