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The FluoTRAM project enhances fluorescence microscopy by integrating transient absorption spectroscopy to capture the dynamics of molecules from their initial excitation state to their final emissive state. This innovative technique provides valuable insights into molecular behavior in biological samples, improving und…
Fluorescence microscopy is an indispensable tool in many areas of research.
In life sciences it has been perfected for biological sample imaging either by its autofluorescence or using fluorescent markers such as dyes or fluorescent proteins.
It is thus possible to localize molecules in cells, obtaining wealth of information on their dynamics and environment.
Despite its power, the fluorescence detection is, by its nature, limited to the information on the final, emissive state of the molecules…
UNIVERZITA KARLOVA
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