CORDIS Project
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This project aims to enhance the understanding of ligand-gated ion channels by combining single molecule fluorescence detection with single channel electrophysiology. This approach will allow for simultaneous observation of ligand binding and channel gating, providing insights into the mechanisms of neurotransmitter ac…
Understanding the dynamics of ligand-gated ion channels is essential for understanding synaptic processes and neuronal signal integration (neurotransmitter gated channels) and intracellular signalling (cyclic nucleotide gated channels).
Here we will combine single molecule fluorescence detection, with single channel electrophysiology, to detect ligand-binding and follow subsequent channel gating simultaneously.
Single-channel patch-clamp alone can only measure two experimental states and their d…
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