CORDIS Project
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This project investigates the relationship between enzyme structure and function at the single molecule level using advanced optical and force spectroscopy techniques. By analyzing the effects of temperature and mechanical tension on enzyme activity, the research aims to provide insights into the dynamic behavior of en…
The relation between structure and functions is a central topic in enzymology. I propose to study that question at the single molecule level employing a novel optical and force spectroscopy.
The enzyme will be linked to a glass surface employing PEG-Biotin-Streptavidin interaction.
The location of a single enzyme will be determined by a dye attached to it.
The substrate of the reaction, while being non-fluorescent, will be chosen so that its enzymatic reaction product is fluorescent.
The time sp…
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
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