CORDIS Project
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The project focuses on developing stand-alone enzymes from complex protein structures by utilizing advanced modeling techniques to predict beneficial mutations. This approach aims to enhance enzyme efficiency for biosynthetic applications while simplifying the engineering process.
Natural enzymes have evolved to perform their functions under complex selective pressures, being capable of accelerating reactions by several orders of magnitude.
In particular, heteromeric enzyme complexes catalyze an enormous array of useful reactions that are often allosterically regulated by different protein partners.
Unfortunately, the underlying physical principles of this regulation are still under debate, which makes the alteration of enzyme structure towards useful isolated subunits a…
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