CORDIS Project
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This project investigates the regulation of DnaA, a protein crucial for DNA replication in bacteria. By characterizing mutants and using advanced microscopy techniques, it aims to uncover how metabolic levels influence DNA replication processes.
In all organisms, the duplication of the genetic material is a coordinated and precise event.
Chromosomes are replicated only once per cell cycle and when over or under replication take place, cell proliferation is severely hindered.
In nearly all bacteria, the coordination of the chromosomal replication is dictated by DnaA.
In Escherichia coli, DnaA initiates the replication by binding DNA sequences in the origin of replication (oriC).
DnaA can only initiate DNA replication when is bound to ATP…
ROSKILDE UNIVERSITET
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