CORDIS Project
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This project utilizes a novel single-molecule technology to study covalent reactions in cells. By measuring ionic current changes through engineered protein pores, it aims to reveal detailed reaction kinetics and develop new cellular sensors.
A label-free single-molecule technology developed the PI's laboratory will be exploited to elucidate covalent chemistry of relevance to the cell.
The approach uses an engineered protein pore that passes a non-perturbing current carried by aqueous ions.
Covalent bond making and breaking events within this nanoreactor are registered as step changes in the ionic current that reveal the kinetics of each reaction step.
No perturbing reagents, such as fluorophores, are required.
Single-molecule chemis…
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
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