CORDIS Project
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This project develops a novel method to analyze cell-free nucleosomes in plasma, providing insights into the transcriptional states of various cell types. By utilizing advanced probabilistic modeling, it aims to link circulating chromatin profiles to disease processes, enhancing non-invasive diagnostics and monitoring…
Genomic DNA is packaged by histone proteins that carry a multitude of post-translational modifications, which reflect cellular transcriptional states.
When cells die, fragmented chromatin retaining histone modifications is released to the circulation.
Recently, we pioneered chromatin immunoprecipitation of cell-free nucleosomes carrying active chromatin marks followed by sequencing (cfChIP-seq).
Our results show that cfChIP-seq provides multidimensional epigenetic information and that the plasma…
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