CORDIS Project
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This project focuses on using solid-state nuclear magnetic resonance to study the structures of bacterial nanomachines, specifically type 3 secretion systems and pili. By improving structural analysis techniques, it aims to uncover the atomic details of these critical components in pathogenic bacteria.
Supramolecular assemblies – formed by the self-assembly of hundreds of protein subunits – are part of bacterial nanomachines involved in key cellular processes.
Important examples in pathogenic bacteria are pili and type 3 secretion systems (T3SS) that mediate adhesion to host cells and injection of virulence proteins.
Structure determination at atomic resolution of such assemblies by standard techniques such as X-ray crystallography or solution NMR is severely limited:
Intact T3SSs or pili cann…
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