CORDIS Project
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This project investigates enzyme promiscuity, focusing on the alkaline phosphatase superfamily. It aims to understand how enzymes can catalyze multiple reactions efficiently, using techniques like linear free energy relationships and site-directed mutagenesis to explore the mechanisms behind this behavior.
Enzymes are the catalysts which allow important chemical reactions to occur on biologically relevant time-scales, and the current understanding of enzymes assumes that each enzyme is specifically tailored for a single reaction.
Enzyme promiscuity refers to an enzyme’s ability to catalyze chemical transformations other than its native reactions.
The identification of an increasing number of promiscuous enzymes challenges the classical “one enzyme, one activity” dogma.The notion that a single enzy…
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